OptiPrep™ Density Gradient Solutions for Mammalian Organelles

OptiPrepTM The ideal density gradient medium for isolation of cells

Actual endotoxin levels in each batch are usually measured at < 0.13 EU/ml. Unlike manufacturers of other density gradient media, Axis-Shield do not have to offer special, more expensive, low endotoxin-containing products; all Axis-Shield Density Gradient Media have always been, and continue to be, produced to the same high rigorous standards. For every batch produced, a Certificate of Analysis...

OptiPrepTM The ideal density gradient medium for isolation of macromolecules and lipoproteins

The flotation of the different classes of human plasma lipoproteins in a centrifugal field by the sequential elevation of the density of the plasma by dissolution of KBr or NaCl/KBr mixtures has been widely used over many years. The operation is routinely carried out in a fixed-angle rotor and takes at least three days. In spite of its cumbersome nature, the technique is still widely used today.

OptiPrepTM The ideal density gradient medium for purification and analysis of viruses

OptiPrepTM The ideal density gradient medium for the purification of plasma membrane and domains

OptiPrepTM offers three gradient formats for the isolation of the plasma membrane (PM). Continuous or discontinuous gradients (1-3h) Continuous gradients (12-16h) Flotation through discontinuous gradients OptiPrepTM offers two approaches to the purification of lipid-rich micro domains. The first to be developed involves the isolation of caveolae from a sonicated total plasma membrane fraction. ...

New conditions on ground state solutions for Hamiltonian elliptic systems with gradient terms

This paper is concerned with the following elliptic system:$$ left{ begin{array}{ll} -triangle u + b(x)nabla u + V(x)u=g(x, v), -triangle v - b(x)nabla v + V(x)v=f(x, u), end{array} right. $$ for $x in {R}^{N}$, where $V $, $b$ and $W$ are 1-periodic in $x$, and $f(x,t)$, $g(x,t)$ are super-quadratic. In this paper, we give a new technique to show the boundedness of Cerami sequences and estab...